Drug toxicity profiling of a Saccharomyces cerevisiae deubiquitinase deletion panel shows that acetaminophen mimics tyrosine.
Identifieur interne : 000616 ( Main/Exploration ); précédent : 000615; suivant : 000617Drug toxicity profiling of a Saccharomyces cerevisiae deubiquitinase deletion panel shows that acetaminophen mimics tyrosine.
Auteurs : Angelina Huseinovic [Pays-Bas] ; Marc Van Dijk [Pays-Bas] ; Nico P E. Vermeulen [Pays-Bas] ; Fred Van Leeuwen [Pays-Bas] ; Jan M. Kooter [Pays-Bas] ; J Chris Vos [Pays-Bas]Source :
- Toxicology in vitro : an international journal published in association with BIBRA [ 1879-3177 ] ; 2018.
Descripteurs français
- KwdFr :
- Acétaminophène (analogues et dérivés), Acétaminophène (composition chimique), Acétaminophène (effets indésirables), Aminophénols (composition chimique), Aminophénols (effets indésirables), Analgésiques non narcotiques (composition chimique), Analgésiques non narcotiques (effets indésirables), Analyse de regroupements (MeSH), Délétion de gène (MeSH), Enzymes de désubiquitinylation (génétique), Enzymes de désubiquitinylation (métabolisme), Haploïdie (MeSH), Isoenzymes (génétique), Isoenzymes (métabolisme), Résistance bactérienne aux médicaments (MeSH), Saccharomyces cerevisiae (croissance et développement), Saccharomyces cerevisiae (effets des médicaments et des substances chimiques), Saccharomyces cerevisiae (enzymologie), Saccharomyces cerevisiae (génétique), Structure moléculaire (MeSH), Stéréoisomérie (MeSH), Tests de toxicité (méthodes), Tyrosine (analogues et dérivés), Tyrosine (composition chimique), Tyrosine (métabolisme), Viabilité microbienne (effets des médicaments et des substances chimiques).
- MESH :
- analogues et dérivés : Acétaminophène, Tyrosine.
- composition chimique : Acétaminophène, Aminophénols, Analgésiques non narcotiques, Tyrosine.
- croissance et développement : Saccharomyces cerevisiae.
- effets des médicaments et des substances chimiques : Saccharomyces cerevisiae, Viabilité microbienne.
- effets indésirables : Acétaminophène, Aminophénols, Analgésiques non narcotiques.
- enzymologie : Saccharomyces cerevisiae.
- génétique : Enzymes de désubiquitinylation, Isoenzymes, Saccharomyces cerevisiae.
- métabolisme : Enzymes de désubiquitinylation, Isoenzymes, Tyrosine.
- méthodes : Tests de toxicité.
- Analyse de regroupements, Délétion de gène, Haploïdie, Résistance bactérienne aux médicaments, Structure moléculaire, Stéréoisomérie.
English descriptors
- KwdEn :
- Acetaminophen (adverse effects), Acetaminophen (analogs & derivatives), Acetaminophen (chemistry), Aminophenols (adverse effects), Aminophenols (chemistry), Analgesics, Non-Narcotic (adverse effects), Analgesics, Non-Narcotic (chemistry), Cluster Analysis (MeSH), Deubiquitinating Enzymes (genetics), Deubiquitinating Enzymes (metabolism), Drug Resistance, Bacterial (MeSH), Gene Deletion (MeSH), Haploidy (MeSH), Isoenzymes (genetics), Isoenzymes (metabolism), Microbial Viability (drug effects), Molecular Structure (MeSH), Saccharomyces cerevisiae (drug effects), Saccharomyces cerevisiae (enzymology), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (growth & development), Stereoisomerism (MeSH), Toxicity Tests (methods), Tyrosine (analogs & derivatives), Tyrosine (chemistry), Tyrosine (metabolism).
- MESH :
- chemical , adverse effects : Acetaminophen, Aminophenols, Analgesics, Non-Narcotic.
- chemical , analogs & derivatives : Acetaminophen, Tyrosine.
- chemical , chemistry : Acetaminophen, Aminophenols, Analgesics, Non-Narcotic, Tyrosine.
- chemical , genetics : Deubiquitinating Enzymes, Isoenzymes.
- chemical , metabolism : Deubiquitinating Enzymes, Isoenzymes, Tyrosine.
- drug effects : Microbial Viability, Saccharomyces cerevisiae.
- enzymology : Saccharomyces cerevisiae.
- genetics : Saccharomyces cerevisiae.
- growth & development : Saccharomyces cerevisiae.
- methods : Toxicity Tests.
- Cluster Analysis, Drug Resistance, Bacterial, Gene Deletion, Haploidy, Molecular Structure, Stereoisomerism.
Abstract
Post-translational protein modification by addition or removal of the small polypeptide ubiquitin is involved in a range of critical cellular processes, like proteasomal protein degradation, DNA repair, gene expression, internalization of membrane proteins, and drug sensitivity. We recently identified genes important for acetaminophen (APAP) toxicity in a comprehensive screen and our findings suggested that a small set of yeast strains carrying deletions of ubiquitin-related genes can be informative for drug toxicity profiling. In yeast, approximately 20 different deubiquitinating enzymes (DUBs) have been identified, of which only one is essential for viability. We investigated whether the toxicity profile of DUB deletion yeast strains would be informative about the toxicological mode of action of APAP. A set of DUB deletion strains was tested for sensitivity and resistance to a diverse series of compounds, including APAP, quinine, ibuprofen, rapamycin, cycloheximide, cadmium, peroxide and amino acids and a cluster analysis was performed. Most DUB deletion strains showed an altered growth pattern when exposed to these compounds by being either more sensitive or more resistant than WT. Toxicity profiling of the DUB strains revealed a remarkable overlap between the amino acid tyrosine and acetaminophen (APAP), but not its stereoisomer AMAP. Furthermore, co-exposure of cells to both APAP and tyrosine showed an enhancement of the cellular growth inhibition, suggesting that APAP and tyrosine have a similar mode of action.
DOI: 10.1016/j.tiv.2017.12.007
PubMed: 29258884
Affiliations:
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(chemistry)</term><term>Cluster Analysis (MeSH)</term><term>Deubiquitinating Enzymes (genetics)</term><term>Deubiquitinating Enzymes (metabolism)</term><term>Drug Resistance, Bacterial (MeSH)</term><term>Gene Deletion (MeSH)</term><term>Haploidy (MeSH)</term><term>Isoenzymes (genetics)</term><term>Isoenzymes (metabolism)</term><term>Microbial Viability (drug effects)</term><term>Molecular Structure (MeSH)</term><term>Saccharomyces cerevisiae (drug effects)</term><term>Saccharomyces cerevisiae (enzymology)</term><term>Saccharomyces cerevisiae (genetics)</term><term>Saccharomyces cerevisiae (growth & development)</term><term>Stereoisomerism (MeSH)</term><term>Toxicity Tests (methods)</term><term>Tyrosine (analogs & derivatives)</term><term>Tyrosine (chemistry)</term><term>Tyrosine (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acétaminophène (analogues et dérivés)</term><term>Acétaminophène (composition chimique)</term><term>Acétaminophène (effets indésirables)</term><term>Aminophénols (composition chimique)</term><term>Aminophénols (effets indésirables)</term><term>Analgésiques non narcotiques (composition chimique)</term><term>Analgésiques non narcotiques (effets indésirables)</term><term>Analyse de regroupements (MeSH)</term><term>Délétion de gène (MeSH)</term><term>Enzymes de désubiquitinylation (génétique)</term><term>Enzymes de désubiquitinylation (métabolisme)</term><term>Haploïdie (MeSH)</term><term>Isoenzymes (génétique)</term><term>Isoenzymes (métabolisme)</term><term>Résistance bactérienne aux médicaments (MeSH)</term><term>Saccharomyces cerevisiae (croissance et développement)</term><term>Saccharomyces cerevisiae (effets des médicaments et des substances chimiques)</term><term>Saccharomyces cerevisiae (enzymologie)</term><term>Saccharomyces cerevisiae (génétique)</term><term>Structure moléculaire (MeSH)</term><term>Stéréoisomérie (MeSH)</term><term>Tests de toxicité (méthodes)</term><term>Tyrosine (analogues et dérivés)</term><term>Tyrosine (composition chimique)</term><term>Tyrosine (métabolisme)</term><term>Viabilité microbienne (effets des médicaments et des substances chimiques)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="adverse effects" xml:lang="en"><term>Acetaminophen</term><term>Aminophenols</term><term>Analgesics, Non-Narcotic</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Acetaminophen</term><term>Tyrosine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Acetaminophen</term><term>Aminophenols</term><term>Analgesics, Non-Narcotic</term><term>Tyrosine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Deubiquitinating Enzymes</term><term>Isoenzymes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Deubiquitinating Enzymes</term><term>Isoenzymes</term><term>Tyrosine</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Acétaminophène</term><term>Tyrosine</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Acétaminophène</term><term>Aminophénols</term><term>Analgésiques non narcotiques</term><term>Tyrosine</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Microbial Viability</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Saccharomyces cerevisiae</term><term>Viabilité microbienne</term></keywords><keywords scheme="MESH" qualifier="effets indésirables" xml:lang="fr"><term>Acétaminophène</term><term>Aminophénols</term><term>Analgésiques non narcotiques</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Enzymes de désubiquitinylation</term><term>Isoenzymes</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Toxicity Tests</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Enzymes de désubiquitinylation</term><term>Isoenzymes</term><term>Tyrosine</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Tests de toxicité</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cluster Analysis</term><term>Drug Resistance, Bacterial</term><term>Gene Deletion</term><term>Haploidy</term><term>Molecular Structure</term><term>Stereoisomerism</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de regroupements</term><term>Délétion de gène</term><term>Haploïdie</term><term>Résistance bactérienne aux médicaments</term><term>Structure moléculaire</term><term>Stéréoisomérie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Post-translational protein modification by addition or removal of the small polypeptide ubiquitin is involved in a range of critical cellular processes, like proteasomal protein degradation, DNA repair, gene expression, internalization of membrane proteins, and drug sensitivity. We recently identified genes important for acetaminophen (APAP) toxicity in a comprehensive screen and our findings suggested that a small set of yeast strains carrying deletions of ubiquitin-related genes can be informative for drug toxicity profiling. In yeast, approximately 20 different deubiquitinating enzymes (DUBs) have been identified, of which only one is essential for viability. We investigated whether the toxicity profile of DUB deletion yeast strains would be informative about the toxicological mode of action of APAP. A set of DUB deletion strains was tested for sensitivity and resistance to a diverse series of compounds, including APAP, quinine, ibuprofen, rapamycin, cycloheximide, cadmium, peroxide and amino acids and a cluster analysis was performed. Most DUB deletion strains showed an altered growth pattern when exposed to these compounds by being either more sensitive or more resistant than WT. Toxicity profiling of the DUB strains revealed a remarkable overlap between the amino acid tyrosine and acetaminophen (APAP), but not its stereoisomer AMAP. Furthermore, co-exposure of cells to both APAP and tyrosine showed an enhancement of the cellular growth inhibition, suggesting that APAP and tyrosine have a similar mode of action.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29258884</PMID><DateCompleted><Year>2018</Year><Month>09</Month><Day>18</Day></DateCompleted><DateRevised><Year>2018</Year><Month>09</Month><Day>18</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-3177</ISSN><JournalIssue CitedMedium="Internet"><Volume>47</Volume><PubDate><Year>2018</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Toxicology in vitro : an international journal published in association with BIBRA</Title><ISOAbbreviation>Toxicol In Vitro</ISOAbbreviation></Journal><ArticleTitle>Drug toxicity profiling of a Saccharomyces cerevisiae deubiquitinase deletion panel shows that acetaminophen mimics tyrosine.</ArticleTitle><Pagination><MedlinePgn>259-268</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0887-2333(17)30373-9</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.tiv.2017.12.007</ELocationID><Abstract><AbstractText>Post-translational protein modification by addition or removal of the small polypeptide ubiquitin is involved in a range of critical cellular processes, like proteasomal protein degradation, DNA repair, gene expression, internalization of membrane proteins, and drug sensitivity. We recently identified genes important for acetaminophen (APAP) toxicity in a comprehensive screen and our findings suggested that a small set of yeast strains carrying deletions of ubiquitin-related genes can be informative for drug toxicity profiling. In yeast, approximately 20 different deubiquitinating enzymes (DUBs) have been identified, of which only one is essential for viability. We investigated whether the toxicity profile of DUB deletion yeast strains would be informative about the toxicological mode of action of APAP. A set of DUB deletion strains was tested for sensitivity and resistance to a diverse series of compounds, including APAP, quinine, ibuprofen, rapamycin, cycloheximide, cadmium, peroxide and amino acids and a cluster analysis was performed. Most DUB deletion strains showed an altered growth pattern when exposed to these compounds by being either more sensitive or more resistant than WT. Toxicity profiling of the DUB strains revealed a remarkable overlap between the amino acid tyrosine and acetaminophen (APAP), but not its stereoisomer AMAP. Furthermore, co-exposure of cells to both APAP and tyrosine showed an enhancement of the cellular growth inhibition, suggesting that APAP and tyrosine have a similar mode of action.</AbstractText><CopyrightInformation>Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Huseinovic</LastName><ForeName>Angelina</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>AIMMS, Division of Molecular Toxicology, Department of Chemistry and Pharmaceutical Sciences, VU University Amsterdam, 1081 HZ Amsterdam, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>van Dijk</LastName><ForeName>Marc</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>AIMMS, Division of Molecular Toxicology, Department of Chemistry and Pharmaceutical Sciences, VU University Amsterdam, 1081 HZ Amsterdam, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vermeulen</LastName><ForeName>Nico P E</ForeName><Initials>NPE</Initials><AffiliationInfo><Affiliation>AIMMS, Division of Molecular Toxicology, Department of Chemistry and Pharmaceutical Sciences, VU University Amsterdam, 1081 HZ Amsterdam, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>van Leeuwen</LastName><ForeName>Fred</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Division of Gene Regulation, Netherlands Cancer Institute, Amsterdam 1066 CX, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kooter</LastName><ForeName>Jan M</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>AIMMS, Department of Molecular Cell Biology, Section Genetics, VU University Amsterdam, 1081 HZ Amsterdam, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vos</LastName><ForeName>J Chris</ForeName><Initials>JC</Initials><AffiliationInfo><Affiliation>AIMMS, Division of Molecular Toxicology, Department of Chemistry and Pharmaceutical Sciences, VU University Amsterdam, 1081 HZ Amsterdam, The Netherlands. Electronic address: j.c.vos@vu.nl.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>12</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Toxicol In Vitro</MedlineTA><NlmUniqueID>8712158</NlmUniqueID><ISSNLinking>0887-2333</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000627">Aminophenols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018712">Analgesics, Non-Narcotic</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007527">Isoenzymes</NameOfSubstance></Chemical><Chemical><RegistryNumber>362O9ITL9D</RegistryNumber><NameOfSubstance UI="D000082">Acetaminophen</NameOfSubstance></Chemical><Chemical><RegistryNumber>42HK56048U</RegistryNumber><NameOfSubstance UI="D014443">Tyrosine</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.19.12</RegistryNumber><NameOfSubstance UI="D000072017">Deubiquitinating Enzymes</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000082" MajorTopicYN="N">Acetaminophen</DescriptorName><QualifierName UI="Q000009" MajorTopicYN="Y">adverse effects</QualifierName><QualifierName UI="Q000031" MajorTopicYN="N">analogs & derivatives</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000627" MajorTopicYN="N">Aminophenols</DescriptorName><QualifierName UI="Q000009" MajorTopicYN="N">adverse effects</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018712" MajorTopicYN="N">Analgesics, Non-Narcotic</DescriptorName><QualifierName UI="Q000009" MajorTopicYN="Y">adverse effects</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016000" MajorTopicYN="N">Cluster Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000072017" MajorTopicYN="N">Deubiquitinating Enzymes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D024881" MajorTopicYN="N">Drug Resistance, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017353" MajorTopicYN="N">Gene Deletion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006238" MajorTopicYN="N">Haploidy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007527" MajorTopicYN="N">Isoenzymes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D050296" MajorTopicYN="N">Microbial Viability</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015394" MajorTopicYN="N">Molecular Structure</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013237" MajorTopicYN="N">Stereoisomerism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018675" MajorTopicYN="N">Toxicity Tests</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014443" MajorTopicYN="N">Tyrosine</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="N">analogs & derivatives</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">APAP</Keyword><Keyword MajorTopicYN="N">Amino acids</Keyword><Keyword MajorTopicYN="N">Deubiquitinase</Keyword><Keyword MajorTopicYN="N">Starvation</Keyword><Keyword MajorTopicYN="N">Toxicity</Keyword><Keyword MajorTopicYN="N">Tyrosine</Keyword><Keyword MajorTopicYN="N">Ubiquitin</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>08</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>12</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>12</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>12</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>9</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>12</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29258884</ArticleId><ArticleId IdType="pii">S0887-2333(17)30373-9</ArticleId><ArticleId IdType="doi">10.1016/j.tiv.2017.12.007</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pays-Bas</li></country></list><tree><country name="Pays-Bas"><noRegion><name sortKey="Huseinovic, Angelina" sort="Huseinovic, Angelina" uniqKey="Huseinovic A" first="Angelina" last="Huseinovic">Angelina Huseinovic</name></noRegion><name sortKey="Kooter, Jan M" sort="Kooter, Jan M" uniqKey="Kooter J" first="Jan M" last="Kooter">Jan M. Kooter</name><name sortKey="Van Dijk, Marc" sort="Van Dijk, Marc" uniqKey="Van Dijk M" first="Marc" last="Van Dijk">Marc Van Dijk</name><name sortKey="Van Leeuwen, Fred" sort="Van Leeuwen, Fred" uniqKey="Van Leeuwen F" first="Fred" last="Van Leeuwen">Fred Van Leeuwen</name><name sortKey="Vermeulen, Nico P E" sort="Vermeulen, Nico P E" uniqKey="Vermeulen N" first="Nico P E" last="Vermeulen">Nico P E. Vermeulen</name><name sortKey="Vos, J Chris" sort="Vos, J Chris" uniqKey="Vos J" first="J Chris" last="Vos">J Chris Vos</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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